Histological stains (specialty)

Record Type:Technique

Validation:,Non-GLP

The Hematoxylin and Eosin (H&E) method is the routine, recognized stain used within histology laboratories; however, many other special tinctorial stains are used on specific tissue or cellular components to aid diagnosis of toxicological or pathological changes.

Special Stain Method:

Specialized Stain

for the demonstration of:

Alcian Blue

Acid Mucins

Alcian Blue PAS

Acid, Neutral and Combined Mucins

Aldehyde Fuchsin (Gomori’s)

Lipofuscins

Aldehyde Fuchsin (modified)

Pancreatic Islet Cells

Alizarin Red

Calcium

Carbol Chromotrope

Eosinophil Leucocytes

Chromotrope Aniline Blue

Hyaline Droplets

Congo Red

Amyloid

Dunn-Thompson

Haemoglobin

Ferric Hematoxylin

Phospholipids

Feulgen

DNA

Fluro Jade B (fluorescence)

Degenerating Neurones

Fouchet’s

Bile Pigment

Giemsa

Parasites

Glees Marsland (Bielschowsky)

Central and Peripheral Nerves

Goldner Trichrome

Mineralised and Osteoid Bone

Gordon and Sweets

Reticulin

Gram

Bacteria

Gridley’s

Fungi

Grimelius

Argyrophil Cells

Grocott’s (Gomori)

真菌和Basement Membranes

Luxol Fast Blue and Cresyl Fast Violet

Myelin and Nissl substance

Martius Scarlet Blue

Fibrin, Connective Tissue

Masson Fontana

Melanin

Masson’s Trichrome

Connective Tissue (Collagen)

Methyl Green Pyronin

DNA and RNA

Movat’s Pentachrome

Connective Tissue (Collagen)

Nile Red (fluorescence)

Lipids

Okajima

Haemoglobin

Oil Red O

Lipid

Periodic Acid Schiff (PAS) Diastase

Glycogen

Periodic Acid Schiff (PAS) Orange G

Acidophil Cells

Periodic Acid Schiff (PAS)

Periodate Carbohydrates,

Basement Membranes

Perl’s

Ferric Iron

Perl’s Prussian Blue

Ferric Iron

Phloxine Tartrazine

Viral Inclusion Bodies

PTAH (Phosophotungstic acid-haematoxylin)

Muscle Striations

Schmorl’s Ferricyanide

Lipofuscin

Sirius Red

Amyloid and Eosinophils

Solochrome Cyanin

Myelin

Toluidine Blue

Mast Cells, Cartlidge Matrix, Acid Mucins

Van Gieson (Picro-Fuchsin)

Connective Tissue

Verhoeff Van Gieson

Elastic Fibres, Connective Tissue (Collagen)

Von Kossa

Mineralised Bone and Osteoid

Warthin-Starry

Spirochetes

Ziehl-Neelsen

Lipofuscin and Ceroid

Ziehl-Neelsen (modified)

Mycobacterium, TB, Leprae

Grocott's Special Stain
Grocott’s Special Stain – demonstrating fungal hyphae
Goldner Trichome Special Stain – demonstrating mineralized and osteoid bone
Goldner Trichome Special Stain – demonstrating mineralized and osteoid bone
Gordon and Sweets Special Stain – demonstrating reticulin in the liver
Gordon and Sweets Special Stain – demonstrating reticulin in the liver
Martius Scarlet Blue Special Stain – demonstrating connective tissue
Martius Scarlet Blue Special Stain – demonstrating connective tissue
Glees Marsland Special Stain – demonstrating neurones in the brain
Glees Marsland Special Stain – demonstrating neurones in the brain
Perl’s Special Stain – demonstrating ferric iron in the liver
Perl’s Special Stain – demonstrating ferric iron in the liver

Industry Market

Pharma,Crop,Chemical

Phase

Nonclinical