Skin irritation & corrosion studies

Record Type:Evaluation

Validation:GLP,Non-GLP

Models:老鼠,Rabbit,NHP,Other Model

The Skin Irritation Test provides a mechanistically relevant measurement of cell viability in reconstructed human epidermis tissues after a single topically applied chemical treatment.

Tests are performed using in vitro 3D human skin tissue models that closely mimic the human epidermis, such as EpiDerm™ and Episkin™ models, which consist of human epidermal keratinocytes.

刺激性化学物质are identified by their ability to decrease cell viability below defined threshold levels.

Cell viability is measured by enzymatic conversion of the vital dye MTT into a blue formazan salt that is quantitatively measured. Colored chemicals can also be assessed using high-performance liquid chromatography (HPLC).

Also see OECD 439.

Administration Routes

Dermal

Industry Market

Crop,Chemical,Pharma,Device