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OECD 439: In vitro skin irritation (EpiSkin™ / EpiDerm™)
Toxicology, In Vitro Alternate
Skin irritation & corrosion studies
The Skin Irritation Test provides a mechanistically relevant measurement of cell viability in reconstructed human epidermis tissues after a single topically applied chemical treatment.
Tests are performed using in vitro 3D human skin tissue models that closely mimic the human epidermis, such as EpiDerm™ and Episkin™ models, which consist of human epidermal keratinocytes.
刺激性化学物质are identified by their ability to decrease cell viability below defined threshold levels.
Cell viability is measured by enzymatic conversion of the vital dye MTT into a blue formazan salt that is quantitatively measured. Colored chemicals can also be assessed using high-performance liquid chromatography (HPLC).
About our nonclinical animal research models: We provide the utmost care for our research animals and actively pursue advances in animal welfare. As well as ensuring the highest scientific, ethical and regulatory standards, selection of appropriate animal research models for development research is critical to developing safe and effective treatments, devices and products that protect and save lives. Just as we have ethical committee oversight for human trials, we have ethical committees overseeing all aspects of our global animal care and use programs. All of our core, nonclinical facilities are accredited by AAALAC.Learn more about our commitment to replacing, reusing and refining nonclinical research models.