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OECD 442C: Direct peptide reactivity assay (DPRA) for skin sensitization
遗传，急性, In Vitro Alternate
OECD 490：使用胸苷激酶基因（MLA TK，体外哺乳动物）的细胞基因突变试验
The in vitro Mammalian Cell Gene Mutation Test is used to detect gene mutations induced by chemicals. The cell line L5178Y TK+/--3.7.2C mouse lymphoma is used for the assay to measure mutations in the thymidine kinase gene. Cells deficient in thymidine kinase (TK) due to the mutation TK+/- to TK-/- are resistant to the cytostatic effects of the pyrimidine analogue trifluorothymidine (TFT).
Normal cells can produce the TK enzyme, therefore are sensitive to TFT, which causes the inhibition of cellular metabolism and prevents further cell division. Whereas mutant cells without the functional TK gene can proliferate in the presence of TFT. The mutant frequency is derived from the number of mutant colonies in the selective medium and the viability. The deficiency in TK can result from genetic events affecting the TK gene including both gene mutations (point mutations, frame-shift mutations, small deletions, etc.) and chromosomal events (large deletions, chromosome rearrangements and mitotic recombination).
Related Capabilities
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经合组织405 / OCSPP 870.2400: Acute eye irritation/corrosion
遗传，急性 -
OECD 476：细胞基因突变试验（HPRT基因，体外哺乳动物）
毒理学，遗传学 -
OECD 489: Alkaline comet assay (in vivo mammalian)
毒理学，遗传学 -
OECD 473: Chromosome aberration test (in vitro mammalian)
毒理学，遗传学 -
微核机械后续行动
毒理学，遗传学 -
经合组织475：骨髓染色体畸变试验（哺乳动物）
毒理学，遗传学 -
OECD 474: Erythrocyte micronucleus test (in vivo mammalian)
毒理学，遗传学 -
OECD 490：使用胸苷激酶基因（MLA TK，体外哺乳动物）的细胞基因突变试验
毒理学，遗传学 -
OECD 471：细菌反向突变试验（AME）
毒理学，遗传学 -
经合组织488：转基因啮齿动物体细胞和生殖细胞基因突变测定（Muta™小鼠）
毒理学，遗传学 -
经合组织404：急性皮肤刺激
毒理学，遗传学, Acute -
OECD 487: Cell micronucleus test (in vitro mammalian)
毒理学，遗传学 -
Toxtracker®Mastay.
毒理学，遗传学 -
Erythrocyte pig-a gene mutation assay (rodent)
毒理学，遗传学 -
Toxicity studies: Acute
遗传，急性 -
局部淋巴结测定（LLNA）
遗传，专业，急性 -
Eye irritation
遗传，体外交替，急性 -
Skin irritation & corrosion studies
遗传，急性
About our nonclinical animal research models: We provide the utmost care for our research animals and actively pursue advances in animal welfare. As well as ensuring the highest scientific, ethical and regulatory standards, selection of appropriate animal research models for development research is critical to developing safe and effective treatments, devices and products that protect and save lives. Just as we have ethical committee oversight for human trials, we have ethical committees overseeing all aspects of our global animal care and use programs. All of our core, nonclinical facilities are accredited by AAALAC.了解有关我们对更换，重新塑造和炼制非临床研究模式的承诺。