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OECD 442C: Direct peptide reactivity assay (DPRA) for skin sensitization
Genetic, Acute, In Vitro Alternate
OECD 476: Cell gene mutation tests (HPRT gene, in vitro mammalian)
The In vitro Mammalian Cell Gene Mutation Test can be used to detect the potential of a chemical to induce gene mutations. The hypoxanthine phosphoribosyl transferase (HPRT) test detects mutations in the endogeneous hypoxanthine-guanine phosphoribosyl transferase gene (Hprt in rodent cells, HPRT in human cells); more specifically, base pair mutations, frameshift mutations and small deletions and insertions. To estimate the mutagenic potential of a substance, treated cells are placed under selective pressure so that only mutant cells can survive. Lack of HPRT activity results in resistance to the toxic analogue 6-thioguanine (6TG). Thus, mutant cells are able to proliferate in the presence of TG, whereas normal cells, which contain HPRT, are not. The most commonly used cells for the HPRT test include the CHO, CHL and V79 lines of Chinese hamster cells, L5178Y mouse lymphoma cells, and TK6 human lymphoblastoid cells.
Related Capabilities
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OECD 489: Alkaline comet assay (in vivo mammalian)
Toxicology, Genetic -
OECD 475: Bone marrow chromosomal aberration test (mammalian)
Toxicology, Genetic -
OECD 474: Erythrocyte micronucleus test (in vivo mammalian)
Toxicology, Genetic -
OECD 488: Transgenic rodent somatic and germ cell gene mutation assays (Muta™Mouse)
Toxicology, Genetic -
OECD 404: Acute skin irritation
Toxicology, Genetic, Acute -
OECD 487: Cell micronucleus test (in vitro mammalian)
Toxicology, Genetic -
ToxTracker® assay
Toxicology, Genetic -
Local lymph node assay (LLNA)
Genetic, Specialty, Acute -
Skin irritation & corrosion studies
Genetic, Acute -
Toxicity studies: Acute
Genetic, Acute -
Eye irritation
Genetic, In Vitro Alternate, Acute -
经合组织405 / OCSPP 870.2400: Acute eye irritation/corrosion
Genetic, Acute -
OECD 476: Cell gene mutation tests (HPRT gene, in vitro mammalian)
Toxicology, Genetic -
Micronucleus mechanistic follow-up
Toxicology, Genetic -
OECD 490: Cell gene mutation tests using the thymidine kinase gene (MLA TK, in vitro mammalian)
Toxicology, Genetic -
OECD 471: Bacterial reverse mutation test (Ames)
Toxicology, Genetic -
Erythrocyte pig-a gene mutation assay (rodent)
Toxicology, Genetic -
OECD 473: Chromosome aberration test (in vitro mammalian)
Toxicology, Genetic
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