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免疫分型与流式细胞术联合应用高通量分析CT26肿瘤中MAPK和JAK-STAT信号
海报
作者：
David Draper, Alden Wong, Philip Lapinski, Sheri Barnes, Maryland Rosenfeld Franklin, and Scott Wise
年份：
2018
AACR年会
Poster: "High-Throughput Analysis of MAPK and JAK-STAT Signaling in CT26 Tumors Using a Combination of Immunophenotyping and Phospho-Flow Cytometry"
简介和背景
- 开发能够在肿瘤微环境中进行基于磷蛋白的细胞信号分析的强大平台对于小分子药物的开发至关重要。目前缺乏可靠的应用，可以同时测量多种磷酸蛋白在肿瘤细胞和免疫细胞离体。This can be crucial because many signaling pathways are commonly involved in both pro- and anti-tumor responses in these two cellular compartments.
- Covance has validated a phospho-flow cytometry-based platform that measures the phospho-protein levels of multiple signaling proteins in separate and distinct subsets simultaneously, which can include solid tumor-derived immune subsets and tumor cells.
- Covance-phospho-flow平台可应用于处理细胞系和异质组织来源细胞培养的分析体外as well as the离体药理学研究中发炎组织的分析。
- 在本演示中，我们演示了如何使用这些服务来分析MAPK和JAK-STAT通路相关激酶体外在培养的MV-4-11急性髓系白血病（AML）细胞和活化的小鼠脾细胞中，以及离体在肿瘤源性CD8+T细胞和肿瘤细胞中均采用CT26结直肠癌模型。