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href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">descubrimiento y lo.据span></span></a> <div> <ul class="tier-3"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>descubrimiento y lo.据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/lead-optimization/lead-optimization-non-glp-toxicology' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/lead-optimization/lead-optimization-non-glp-toxicology.html" data-url="//www.wfliji.com/es/services/lead-optimization/lead-optimization-non-glp-toxicology.html">SEGURIDAD恩incumplimiento德拉斯BPL据span></span></a></li> <li 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href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/services/lead-optimization/nonclinical-imaging.html">Imagenologíapreclínica据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>Imagenologíapreclínica据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/services/lead-optimization/nonclinical-imaging/computed-tomography-ct.html">TomografíaComputaradada.据/a> <a href="//www.wfliji.com/es/services/lead-optimization/nonclinical-imaging/bioluminescence-imaging.html">Bioluminiscencia据/a> <a href="//www.wfliji.com/es/services/lead-optimization/nonclinical-imaging/image-analysis-services.html">进行分析据/a> <a href="//www.wfliji.com/es/services/lead-optimization/nonclinical-imaging/positron-emission-tomography.html">Emisióndediorrones.据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/lead-optimization/radiation' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/lead-optimization/radiation.html" data-url="//www.wfliji.com/es/services/lead-optimization/radiation.html">Radiación.据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/lead-optimization/flow-cytometry' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/lead-optimization/flow-cytometry.html" data-url="//www.wfliji.com/es/services/lead-optimization/flow-cytometry.html">CITOMETRíaFULJO.据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/lead-optimization/vaccines' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/lead-optimization/vaccines.html" data-url="//www.wfliji.com/es/services/lead-optimization/vaccines.html">撤离据span></span></a></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/product-testing' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">pruebas de productos据span></span></a> <div> <ul class="tier-3"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>pruebas de productos据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/product-testing/crop' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/services/product-testing/crop.html">CULTIVOS据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>CULTIVOS据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/services/product-testing/locations.html">Ubicaciones.据/a> <a href="//www.wfliji.com/es/services/product-testing/crop/physical-chemistry.html">Fisicoquimica据/a> <a href="//www.wfliji.com/es/services/product-testing/crop/safety-assessment.html">Seguridad Humana.据/a> <a href="//www.wfliji.com/es/services/product-testing/crop/environmental-safety.html">Seguridad Ambiental.据/a> <a href="//www.wfliji.com/es/services/product-testing/crop/endocrine-disruptors.html">Disruptores endocrinos（DE）据/a> <a href="//www.wfliji.com/es/services/product-testing/crop/regulatory-consultancy.html">Reglamentaciónycomplimiento.据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/product-testing/chemical-testing' }, collect: false, options: { obj: 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href="//www.wfliji.com/es/services/product-testing/chemical-testing/environmental-safety.html">Seguridad Ambiental.据/a> <a href="//www.wfliji.com/es/services/product-testing/chemical-testing/endocrine-disruptors.html">讨厌内科科纳斯据/a> <a href="//www.wfliji.com/es/services/product-testing/chemical-testing/regulatory-consultancy.html">ConsultoríanstsuntosReglemarios据/a> <a href="//www.wfliji.com/es/services/product-testing/chemical-testing/education-center.html">InformAción.据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/product-testing/locations' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/services/product-testing/locations.html">Ubicaciones.据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>Ubicaciones.据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/services/product-testing/locations/greenfield-in.html">格林菲尔德据/a> <a href="//www.wfliji.com/es/services/product-testing/locations/huntingdon-uk.html">亨廷顿据/a> <a href="//www.wfliji.com/es/services/product-testing/locations/shardlow.html">Shardlow据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/product-testing/scientific-spotlights' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/services/product-testing/scientific-spotlights.html">destacadoscientíficos据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>destacadoscientíficos据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/services/product-testing/scientific-spotlights/oecd-229-fish-short-term-reproductive-assay.html">经合组织229.据/a> <a href="//www.wfliji.com/es/services/product-testing/scientific-spotlights/oecd-231-amphibian-metamorphosis-assay.html">经合组织231年据/a> </div></li> </ul> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/pathology' }, collect: false, options: { obj: this }, componentPath: 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href="//www.wfliji.com/es/services/safety-assessment/general-toxicology/nonhuman-primate-studies.html">PNH.据/a> <a href="//www.wfliji.com/es/services/safety-assessment/general-toxicology/ocular-development-services.html">眼镜据/a> <a href="//www.wfliji.com/es/services/preclinical/send.html">发送3,1据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/safety-assessment/genetic-toxicology' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/services/safety-assessment/genetic-toxicology.html">GENÉTICA据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>GENÉTICA据/span></li> <li 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<ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>Comercialización.据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/services/postmarketing/market-access/evidence-generation.html">FerensacióndeVidencia据/a> <a href="//www.wfliji.com/es/services/postmarketing/market-access/field-services.html">SERVICIOS DE坎普据/a> <a href="//www.wfliji.com/es/services/postmarketing/market-access/market-access-consulting.html">ConsultoríadeCesoAl Mercado据/a> <a href="//www.wfliji.com/es/services/postmarketing/market-access/economic-modeling.html">ModeloEconómico.据/a> <a href="//www.wfliji.com/es/services/postmarketing/market-access/payer-insights.html">Precios.据/a> <a href="//www.wfliji.com/es/services/postmarketing/market-access/value-communication.html">COMUNICACION德VALORES据/a> <a href="//www.wfliji.com/es/services/postmarketing/market-access/australia.html">Acceso：亚洲 - 太平洋据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/postmarketing/patient-support' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/services/postmarketing/patient-support.html">Asistencia Al Paciente.据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>Asistencia Al Paciente.据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a 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data-url="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance.html">seguridad y fv.据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>seguridad y fv.据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/aggregate-reporting.html">通知协议据/a> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/automation.html">Automatización.据/a> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/patient-safety-consulting.html">ConsultoríaSobreSeguridad.据/a> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/pharmacovigilance.html">Farmacovigilancia（FV）据/a> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/safety-technology.html">TecnologíadeSeguridad.据/a> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/signal-detection-and-risk-management.html">Detecciónygestiónderiesgos据/a> <a href="//www.wfliji.com/es/services/postmarketing/patient-safety-and-pharmacovigilance/patient-safety-resource-center.html">InformAción.据/a> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/postmarketing/phase-iv-solutions' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/postmarketing/phase-iv-solutions.html" data-url="//www.wfliji.com/es/services/postmarketing/phase-iv-solutions.html">estudios de fase iv据span></span></a></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/device' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">Dispositivos据span></span></a> <div> <ul class="tier-3"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>Dispositivos据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/device/our-strategic-approach' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/device/our-strategic-approach.html" data-url="//www.wfliji.com/es/services/device/our-strategic-approach.html">Enfoque estrategico据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/device/regulatory-consulting' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/device/regulatory-consulting.html" data-url="//www.wfliji.com/es/services/device/regulatory-consulting.html">Reglamentario据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/device/preclinical-experimental-surgery' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/device/preclinical-experimental-surgery.html" data-url="//www.wfliji.com/es/services/device/preclinical-experimental-surgery.html">Preclínica据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/device/clinical-market-approval' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/services/device/clinical-market-approval.html" data-url="//www.wfliji.com/es/services/device/clinical-market-approval.html">Clínica据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/services/device/commercial-post-market-support' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" 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href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="https://es.covance.com/customers.html">客户据span></span></a> <div> <ul class="tier-2"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>客户据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/global-pharma' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/customers/global-pharma.html" data-url="//www.wfliji.com/es/customers/global-pharma.html">Companias farmaceuticas据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 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href="//www.wfliji.com/es/customers/biotech/the-bio-experience.html" data-url="//www.wfliji.com/es/customers/biotech/the-bio-experience.html">社会据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/biotech/approach' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/customers/biotech/the-bio-experience.html" data-url="//www.wfliji.com/es/customers/biotech/the-bio-experience.html">Metodologia据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/biotech/value' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/customers/biotech/the-bio-experience.html" data-url="//www.wfliji.com/es/customers/biotech/the-bio-experience.html">勇气据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/biotech/transition' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/customers/biotech/the-bio-experience.html" data-url="//www.wfliji.com/es/customers/biotech/the-bio-experience.html">Transición.据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/biotech/contact' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/response/LP=1647" data-url="//www.wfliji.com/response/LP=1647">联系方式据span></span></a></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/investigators' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="//www.wfliji.com/es/customers/investigators.html">InvestigoRes deEssayosClínicos据span></span></a> <div> <ul class="tier-3"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>InvestigoRes deEssayosClínicos据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/investigators/dry-ice' }, collect: false, options: { obj: this }, componentPath: 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href="//www.wfliji.com/es/customers/investigators/become-an-investigator.html" data-url="//www.wfliji.com/es/customers/investigators/become-an-investigator.html">Conviértaseen Investigador.据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/investigators/accreditations-and-certifications' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/customers/investigators/accreditations-and-certifications.html" data-url="//www.wfliji.com/es/customers/investigators/accreditations-and-certifications.html">Acreditacionesÿcertificaciones据span></span></a></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 'https://es.covance.com/content/covance/en/customers/investigators/investigator-study-team' }, collect: false, options: { obj: this }, componentPath: 'covance-redesign/components/structure/header'})" href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" data-url="https://es.covance.com/customers/investigators/investigator-study-team.html">递归个人实际的investigación据span></span></a> <div> <ul class="tier-4 wide"> <a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#" class="bar"></a> <li class="h2"><span>递归个人实际的investigación据/span></li> <li class="back"><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/#">volver.据/a></li> <li> <div> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/courier-contact-numbers.html">德是车辆据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/ups-express.html">ETIQUETA：UPS表达至关重要据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/dry-ice.html">Pedido: hielo入股事宜据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/institution-privacy-statement.html">Declaración de privacidad: institución据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/investigator-privacy-statement.html">Declaración de privacidad:调查者据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/study-staff-privacy-statement.html">AccorAcióndevacacidad：个人de estudio据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/packaging-shipping-videos.html">视频：Empaque YEnvío据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/sample-collection-videos.html">视频：Recogida de Muestras据/a> <a href="//www.wfliji.com/es/customers/investigators/investigator-study-team/faqs.html">Preguntas frecuentes据/a> </div></li> </ul> </div></li> </ul> </div></li> <li class="nav"><a onclick="CQ_Analytics.record({event:'followMegaMenu',values: { menuPath: 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class="text-body"> <p><b>日期：据/b>2018年7月据/p> </div> </div> </div> <div class="aem_text aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0"> <div class="text"> <div class="text-body"> <p><b>作者：据/b>David Draper， |博士，科学发展副主任据br></p> </div> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p>我们可以访问artículo destacado sobre tecnología现在向您介绍我们绝对的原则，我们可以访问美国combinación con análisis inmunofenotípico肿瘤。据/p> </div> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p><a href="//www.wfliji.com/solutions/oncology">Pongase en contacto据/a>Con El Equipo Cientifico deOncología帕拉·索引AMásfignaCión。据/p> </div> <div class="text-button"> <a class="btn btn-solid " href="//www.wfliji.com/solutions/oncology">索利特尔信息据/a> </div> </div> </div> <div class="horizontalRule aem-GridColumn--default--9 aem-GridColumn"> <div data-emptytext="HorizontalRule Component" class="cq-placeholder"></div> <div class="covance-component"> <div class="hrule" style="margin-top: 10px;margin-bottom: 10px;"> <hr class="style-one"> </div> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p>我们可以绝对接受我们的治疗aplicación请允许我们的治疗对象científicos请允许我们的治疗对象citometría请允许我们的治疗对象我们的治疗对象células请允许我们的治疗对象infiltración请允许我们的治疗对象células治疗一个肿瘤。我们可以访问artículo destacado sobre tecnología现在向您介绍我们绝对的原则，我们可以访问美国combinación con análisis inmunofenotípico肿瘤。据/p> <p>La PoceDad de Medir ConPrecisiónLOSCAMBIOSINÁMICOSZHACHUESTAINMUNE DENTRO DEL MICROMENDEE肿瘤（MAT）ES基础Al Probar Nuevas Terapias Inmunomoduladoras。Muntras Que LaConfiguracióndemunotipificaciónCobustosEs Neecesaria Para Democtir Subgrupos ConPrecisión，ElmétodoConelQue Se Se SeeastoSParámetrosPuedeAfectarCómoSEPrintentan洛杉矶·奥斯塔斯·索尼斯Una Lecturacomúnes“％decéluluscd45据sup>+据/sup>“Que Mide LaDistribuciónRearivade Cada Subgrupo Como联合国PorcentajedeCélulasinmunes总计。据sup>1，2据/sup>EsteMétodoes valioso porque ayuda a cuantificar el efecto que tiene la terapia en laproporcióndesubgrupos con diferentes actividades pro y antirumorales。POR Ejeallo，UNAUMENO en LaProporcióndeCélulasTC8据sup>+据/sup>Con UnaDisminuciónecareDieReenlascélululaststandadoras indaga que que el Tratamiento ha Reducido laInmunosupresiónMeddaPorcélulaststancadoras en El Mat。据/p> <p>毫无疑问，我们的医学在distribución como única讲座中有局限性。我要把这一切都告诉你。我们研究了CT26和抗mctla -4的抑制因子。我们研究了observó una marcada inhibición和crecimiento肿瘤(图1)。我们研究了acción的机制，我们研究了citometría de flujo和perfilar la distribución de células CD3据sup>+据/sup>y CD11b据sup>+据/sup>德里亚达斯德尔肿瘤（Célulastycélulasmieloides，尊重）Entre El TotaldeCélulasCD45据sup>+据/sup>（VER IMAGEN 2）。甲partir德estos DATOS，ELintérpretepodríallegar一拉德结论EL bloqueo去CTLA-4 produjo短刀未aumento烯LA cantidad德célulasŤcomo的UNAreducciónsimultánea烯célulasmieloides。埃斯托sugeriría阙拉ACTIVIDADmecanística德尔tratamiento ESTA mediada POR UNA扩展连接拉cantidad德células牛逼CD8据sup>+据/sup>antitumorales ASI科莫tambiénUNAcontracción德拉斯célulasmieloides inmunosupresoras。科莫本身demuestra瓦霍，ESTA结论SERIA incorrecta。据/p> </div> </div> </div> <div class="aem_image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--offset--default--0 aem-GridColumn--default--4"> <div class="image"> <img class="image-img" alt="imagen画质1：硒implantaroncélulasCT26subcutáneamenteEN LA axila derecha德拉托内斯的Balb / c。La dosificación de n=10 animales/grupo se inició cuando los tumores estaban establecidos y se monitoreó la evolución del tumor mediante mediciones de calibre. Las dosis de anticuerpos se administraron dos veces por semana antes de la toma de muestras el día 14." src="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/absolute-counts-by-flow-cytometry-maximize-the-accuracy-of-your-immunophenotypic-data-interpretation/_jcr_content/root/responsivegrid_left/image.coreimg.png/1617112523806/1578408169242.png"> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p><cite>imagen画质1：硒implantaroncélulasCT26subcutáneamenteEN LA axila derecha德拉托内斯的Balb / c。拉dosificaciónDE N = 10阿尼马莱斯/ GRUPO SE INICIO宽多洛斯tumores estaban establecidosý本身monitoreóLAevolución德尔肿瘤mediante mediciones德口径。拉斯维加斯dosis德anticuerpos SE administraron DOS veces POR周前注德拉托马日muestras EL DIA 14。据br><br></cite></p> <p>萨尔瓦多cálculo带串联德recuentos absolutos SE suele城市搜救第superar拉limitacióndescrita阿里巴。埃斯特parámetro酰胺CON精密拉infiltración德洛斯subgrupos celulares连接洛杉矶tumoresŸ连接一般本身reporta科莫cantidad总去célulasPOR团结报德马萨。据sup>3、4、5据/sup>我们可以在绝对的基础上，建立一个完整的关系células mieloides/ tumo de tumo no cambió tras la terapia con anti-mCTLA-4(图2)。la disminución observada en la proporción de células mieloides es más很有可能这个原因在这个绝对的例题中células T.这个人如果有重新分类的话他就会在distribución一个对象的子群体中，我们没有必要在一个绝对的小群体中建立一个联合国，但我们可以在expansión或contracción这个不同的小群体中连续运行。据/p> </div> </div> </div> <div class="aem_image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--offset--default--0 aem-GridColumn--default--4"> <div class="image"> <img class="image-img" alt="Imagen 2：Mediciones deDistribuciónvs.CocuentoAbsolutodecélulastycélulasMieloidesen肿瘤CT26。Se hizo联合国análisisestadísticousando una prueba t de stuest（* p <0,05）。" src="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/absolute-counts-by-flow-cytometry-maximize-the-accuracy-of-your-immunophenotypic-data-interpretation/_jcr_content/root/responsivegrid_left/image_copy.coreimg.png/1617112529559/1578408277425.png"> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p><cite>Imagen 2：Mediciones deDistribuciónvs.CocuentoAbsolutodecélulastycélulasMieloidesen肿瘤CT26。硒HIZO未更新的时候通知estadístico山岛UNA prueba吨德学生（* P <0.05）。据br><br></cite></p> <p>埃尔更新的时候通知inmunofenotípicosubsiguiente德macrófagosASOCIADOS一个tumores（MAT）Y subgrupos德célulasŤenfatiza AUNMÁSEL勇气去incluirparámetros德recuento absoluto。科莫本身muestra EN LA imagen画质3，SI边EL更新的时候通知reveló阙拉proporción德MAT M2disminuyóEN EL肿瘤，没有互搏ningún坎比奥连接洛杉矶recuentos absolutos。POR LO短刀，反证人更新的时候通知inicial，洛杉矶recuentos absolutos没有靛阙拉farmacodinámica德MAT M2 contribuye一拉eficacia通用EN埃斯特ESTUDIO。ŸESTA ES UNA结论MÁSfiable basada连接洛杉矶DATOS disponibles。POR Ultimo的，拉terapia CON抗mCTLA-4 produjo联合国aumento恩拉斯维加斯男女统计资料absolutas TANTO德células牛逼CD8据sup>+据/sup>ComodeCélulastauxiliares cd4据sup>+据/sup>．最后，我们把它写下来cómo los recuentos absolutos pueden ser esenciales para cuantificar correctamente la cantidad total de células para subgrupos derivados de tejidos。据/p> </div> </div> </div> <div class="aem_image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--offset--default--0 aem-GridColumn--default--4"> <div class="image"> <img class="image-img" alt="IMAGEN 3：Mediciones德DISTRIBUCION与recuento absoluto德MAT M1 M2Ÿ，células牛逼CD8 + Ycélulasauxiliares CD4 + EN tumores CT26。Se hizo联合国análisisestadísticousando una prueba t de stuest（* p <0,05）。" src="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/absolute-counts-by-flow-cytometry-maximize-the-accuracy-of-your-immunophenotypic-data-interpretation/_jcr_content/root/responsivegrid_left/image_copy_copy.coreimg.png/1617112528503/1578408389463.png"> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p><cite>IMAGEN 3：Mediciones德DISTRIBUCION与recuento absoluto德MAT M1 M2Ÿ，células牛逼CD8 + Ycélulasauxiliares CD4 + EN tumores CT26。硒HIZO未更新的时候通知estadístico山岛UNA prueba吨德学生（* P <0.05）。据br></cite></p> <h3>Metodologíade Rocuento Absoluto据/h3> <p>埃尔更新的时候通知inmunofenotípicosubsiguiente德macrófagosASOCIADOS一个tumores（MAT）Y subgrupos德célulasŤenfatiza AUNMÁSEL勇气去incluirparámetros德recuento absoluto。科莫本身muestra EN LA imagen画质3，SI边EL更新的时候通知reveló阙拉proporción德MAT M2disminuyóEN EL肿瘤，没有互搏ningún坎比奥连接洛杉矶recuentos absolutos。POR LO短刀，反证人更新的时候通知inicial，洛杉矶recuentos absolutos没有靛阙拉farmacodinámica德MAT M2 contribuye一拉eficacia通用EN埃斯特ESTUDIO。ŸESTA ES UNA结论MÁSfiable basada连接洛杉矶DATOS disponibles。POR Ultimo的，拉terapia CON抗mCTLA-4 produjo联合国aumento恩拉斯维加斯男女统计资料absolutas TANTO德células牛逼CD8据sup>+据/sup>ComodeCélulastauxiliares cd4据sup>+据/sup>．最后，我们把它写下来cómo los recuentos absolutos pueden ser esenciales para cuantificar correctamente la cantidad total de células para subgrupos derivados de tejidos。据/p> </div> </div> </div> <div class="aem_image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0"> <div class="image"> <img class="image-img" alt="图4：使用Labcorp绝对计数流量面板测量吸入的样本吸入，绝对计数的样品的体积，绝对活细胞和活CD45 +细胞的浇注策略。" src="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/absolute-counts-by-flow-cytometry-maximize-the-accuracy-of-your-immunophenotypic-data-interpretation/_jcr_content/root/responsivegrid_left/image_copy_copy_copy.coreimg.jpeg/1617112525387/1578408598285.jpeg"> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p><cite>Imagen 4：Estrategia de Gating Para Medir El Volumen de Muestra Aspirada，Recuentos Absolutos deCofélulasVivas，YCélulasCD45+ Usando El Panel de Flujo de Rocuento Absoluto de Covance。据br></cite></p> <p>La imagen 4 ilustra cómo se calculan los recuentos absolutos de células。La región的微囊费as cuantifica的悬臂的微囊费as adquiridas por el citómetro的flujo的中等体积的声音。我们可以把它放在一个可以生存的地方，但是不能放在células外面。Y después de la exlcusión de células muertas, se mida expresión de CD45，根据células的计算进入células CD45的门据sup>+据/sup>vivas。Luego SE Callula Cantidad Total DeCélulas-南姆斯CD45据sup>+据/sup>Detectadas Por ElCitómentrousando la惯例de abajo。PORúltimo，Como Se Conoce La Masa肿瘤有所单位，SE PUEDE VOLVER A Callular El RecuentodeCélulas/ Gramo del肿瘤。据/p> </div> </div> </div> <div class="aem_image aem-GridColumn--default--none aem-GridColumn aem-GridColumn--default--6 aem-GridColumn--offset--default--0"> <div class="image"> <img class="image-img" alt="然后使用下面的公式计算通过细胞仪检测到的CD45 +免疫细胞的总数。PORúltimo，Como Se Conoce La Masa肿瘤有所单位，SE PUEDE VOLVER A Callular El RecuentodeCélulas/ Gramo del肿瘤。" src="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/absolute-counts-by-flow-cytometry-maximize-the-accuracy-of-your-immunophenotypic-data-interpretation/_jcr_content/root/responsivegrid_left/image_copy_copy_copy_540839446.coreimg.png/1617112516579/1578408796056.png"> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <p>萨尔瓦多CONJUNTO德DATOS COMPLETO对ESTUDIO德阿里巴ESTA disponible一个pedido。描述洛杉矶efectos德bloqueo德puntos日控制连接洛杉矶recuentosŸperfila洛杉矶siguientes subgrupos EN tumores CT26;ASI科莫también洛杉矶paneles德flujo阙本身usaron对hacer estas mediciones。据/p> <ul> <li><p>Célulassupresoras erivadas de mieloidesgranulocícas据/p></li> <li><p>Células supresoras derivadas de mieloides monocíticas据/p></li> <li><p>Célulasdendríticas据/p></li> <li><p>MacrófagosAsociados肿瘤（M1 Y M2）据/p></li> <li><p>CélulasB据/p></li> <li><p>CélulasFarminadorasNaturales.据/p></li> <li><p>CélulasŤexterminadoras naturales据/p></li> <li><p>CélulasŤCD8 +据/p></li> <li><p>Célulastauxiliares cd4 +据/p></li> <li><p>CélulasŤreguladoras据/p></li> </ul> <p><a title="联系科学家" href="//www.wfliji.com/response/oncologyrequest?cid=Web_Clk_SAMLOIS_XSEG-ED-2020-Preclinical-Oncology_0320_2354">联系科学家据/a> <a href="//www.wfliji.com/response/oncologyrequest?cid=Web_Clk_SAMLOIS_XSEG-ED-2020-Preclinical-Oncology_0320_2354">Labcorp.据/a>要求完整的数据集或了解更多关于我们的绝对计数服务，以及如何将其应用于您的临床前研究。yaboapp体育官网据/p> </div> </div> </div> <div class="aem_text aem-GridColumn--default--9 aem-GridColumn"> <div class="text"> <div class="text-body"> <h4>参考文献据/h4> <div> <i><br><sup>1据/sup>Kadić， Elma等。“低温保存对多参数单细胞质量细胞分析确定肿瘤标本中免疫细胞亚群划分的影响”BMC免疫学18,1(2017):6。据br><br></i> </div> <p><sup>2据/sup>等。“白细胞介素-21联合PD-1或CTLA-4阻断剂提高小鼠肿瘤模型的抗肿瘤免疫能力”。肿瘤免疫学7,1 (2018):e1377873。据/p> <p><sup>3.据/sup>Muroyama，柚木，等。“立体定向放射增加功能性抑制性调节性T细胞在肿瘤微环境”。癌症免疫学研究5,11（2017）：992。据/p> <p><sup>4.据/sup>Balza，Enrica等人。“TNF和Melphalan的肿瘤递送诱导的治疗性T细胞反应取决于自然杀伤和树突细胞的早期触发。欧洲免疫学杂志47,4（2017）：743-753。据/p> <div> <i><sup>5.据/sup>Buisseret，劳伦斯等人。“肿瘤浸润淋巴细胞组成，组织和PD-1 / PD-L1表达在乳腺癌链接”。Oncoimmunology 6,1（2017）：e1257452。据/i> </div> <div> <i></i> </div> <div> <i>el据a href="//www.wfliji.com/es/commitment/animal-welfare.html">cuidadoŸUSO德阿尼马莱斯据/a>SeEjecutódeConformidadConLas Normativas de BieneStar Animal en Un Centro Acreditado Por La Aaalac ConRevisiónyAprobacióndeCatectodel Iacuc。据/i> <br> </div> </div> </div> </div> <div class="aem_section-menu aem-GridColumn--default--9 aem-GridColumn"> <div class="section-menu"> <div class="nav_section_btn"> <a>menúdesección.据i class="fa fa-list" aria-hidden="true"></i></a> </div> <nav class="nav_section"> <p class="h5">Más destacados en tecnología y modelos据/p> <ul> <li><span><a href="//www.wfliji.com/es/industry-solutions/oncology/preclinical/tumor-spotlights/Bioluminescence-imaging-spotlight.html">ImagenologíaPorBioluminiscencia：Montorización在Vivo de Desarrollo Tumoral，DiseminaCióndaInfermedadY Eficacia del Tratamiento据/a></span></li> <li><span><a 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