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细胞培养
Covance可以生长并扩大您的细胞系以将数百只小鼠植入数百只小鼠或冻结，根据您需要的细胞浓度，如有许多小瓶。我们拥有14只CO2孵化器，三个全尺寸的生物安全柜，两种细胞自动化单元柜台，以及在菜肴，烧瓶，滚筒瓶，旋转瓶和多层烧瓶中生长数百种不同的细胞系的大量经验。
Covance can monitor the cell morphology of your cells in culture using an Olympus CKX53F inverted fluorescence microscope with phase contrast and a 1.4MP CCD camera. View pictures of your cells before, during, and after treatment, over extended periods of time, or at specific time points before implantation to verify that the cells are growing normally.
我们可以随时间监测细胞的生长，并比较不同的细胞变体，在不同培养基中生长的细胞，或用不同处理种植的细胞。我们有两个LUNA自动化单元计数器，可以准确地区分从死细胞并计算每个人口。我们的细胞培养团队具有大量的体验，培养多种类型的细胞，包括肿瘤细胞，原代细胞和神经球。该团队还具有表演菌落形成测定的经验，以检测转化细胞的致瘤潜力以及蛋白质对转化细胞的肿瘤抑制作用in vitro。我们的流式细胞术团队具有使用CFSE染色在混合淋巴细胞反应中刺激的T细胞或T细胞的体验。
Covance has the ability to stain cells and acquire fluorescent images using the Cytation 3 Imaging Plate Reader. Once the images have been captured, we have specialized software that can count nuclei, count live vs dead cells, count stained cells as positive vs. negative, and measure the size, circularity, area, perimeter, and intensity of each color in the images.
Firefly (P. pyralis) luciferase is an enzyme that produces bioluminescence in the presence of D-luciferin. The DNA for the luciferase 2 (luc2) gene, along with a puromycin resistance gene, is incorporated into the genome of the cells using a lentiviral transduction system. While culturing the cells, puromycin is added to the medium to select only the cells with the inserted luciferase gene. Once the luc-enabled cells are injected into a mouse or rat and D-luciferin is administered, the light produced can be detected with Covance's IVIS® Spectrumin vivoimaging system. The in体外services team has experience performing every step of the process from stably transducing cells, to selecting the transduced cells, to measuring the light output of the cells.
细胞毒性/增殖测定广泛用于筛选复合文库中的细胞毒性。如果您有兴趣开发旨在迅速分裂癌细胞的治疗剂，我们可以测试您的细胞毒性化合物。在您投资于药剂之前，我们还可以从最初的高通量药物屏幕中筛选“命中”为不需要的细胞毒性效果。Covance可以测量您选择的化合物的毒性，在您选择的400多条细胞中。我们提供图形和IC50每个细胞系和化合物的值。