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OECD 442C: Direct peptide reactivity assay (DPRA) for skin sensitization
Genetic, Acute, In Vitro Alternate
OECD 489: Alkaline comet assay (in vivo mammalian)
The In vivo Alkaline Comet (single cell gel electrophoresis) Assay is used to identify chemicals that cause DNA damage by detecting DNA strand breaks in cells or nuclei isolated from multiple tissues of animals, usually rodents, that have been exposed to potentially genotoxic material.
The nuclear DNA undergoes electrophoresis under alkaline conditions (>pH 13) and results in structures resembling comets which can be observed by fluorescent microscopy. The amount of DNA breakage is based on migration of the DNA fragments.
The assay can detect single and double stranded breaks which may be repaired, resulting in no persistent effect, may be lethal to the cell, or may be fixed into a mutation resulting in a permanent viable change. They may also lead to chromosomal damage which is also associated with many human diseases including cancer.
Related Capabilities
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经合组织405 / OCSPP 870.2400: Acute eye irritation/corrosion
Genetic, Acute -
OECD 476: Cell gene mutation tests (HPRT gene, in vitro mammalian)
毒理学、遗传 -
OECD 489: Alkaline comet assay (in vivo mammalian)
毒理学、遗传 -
OECD 473: Chromosome aberration test (in vitro mammalian)
毒理学、遗传 -
Micronucleus mechanistic follow-up
毒理学、遗传 -
OECD 475: Bone marrow chromosomal aberration test (mammalian)
毒理学、遗传 -
OECD 474: Erythrocyte micronucleus test (in vivo mammalian)
毒理学、遗传 -
OECD 490: Cell gene mutation tests using the thymidine kinase gene (MLA TK, in vitro mammalian)
毒理学、遗传 -
OECD 471: Bacterial reverse mutation test (Ames)
毒理学、遗传 -
OECD 488: Transgenic rodent somatic and germ cell gene mutation assays (Muta™Mouse)
毒理学、遗传 -
OECD 404: Acute skin irritation
毒理学、遗传, Acute -
OECD 487: Cell micronucleus test (in vitro mammalian)
毒理学、遗传 -
ToxTracker® assay
毒理学、遗传 -
Erythrocyte pig-a gene mutation assay (rodent)
毒理学、遗传 -
Toxicity studies: Acute
Genetic, Acute -
Local lymph node assay (LLNA)
Genetic, Specialty, Acute -
Eye irritation
Genetic, In Vitro Alternate, Acute -
Skin irritation & corrosion studies
Genetic, Acute
About our nonclinical animal research models: We provide the utmost care for our research animals and actively pursue advances in animal welfare. As well as ensuring the highest scientific, ethical and regulatory standards, selection of appropriate animal research models for development research is critical to developing safe and effective treatments, devices and products that protect and save lives. Just as we have ethical committee oversight for human trials, we have ethical committees overseeing all aspects of our global animal care and use programs. All of our core, nonclinical facilities are accredited by AAALAC.Learn more about our commitment to replacing, reusing and refining nonclinical research models.